Exogenous interleukin 2 recruits in vitro lymphokine-activated killer activity by in vivo activated lymphocytes.

摘要: Cryopreserved and thawed lymphocytes can be used instead of fresh to avoid test-to-test variability in studies fluctuations natural killer (NK) lymphokine-activated (LAK) activities as a function time. We investigated the effects 1-h versus 18-h resting on their lytic activities, because process cryopreservation thawing decreases NK LAK activities. Lymphocytes from renal cell cancer patients receiving adoptive immunotherapy were studied. An period led significant increase activity but had no effect activity. The presence 1200 IU/ml interleukin 2 (IL-2) medium 1 h prior during cytotoxicity (CTX) assay increased vivo vitro IL-2-induced This phenomenon has been interpreted IL-2 dependency effector (J. A. Hank, P. C. Kohler, G. Weil-Hillman, N. Rosenthal, K. H. Moore, B. Storer, D. Minkoff, J. Bradshaw, R. Bechhofer, M. Sondel. Cancer Res., 48: 1965–1971, 1988). performed kinetic assess role lymphocyte recruitment these experiments. was tested or absence preincubations CTX assays varying between 0 120 min. These showed that indeed contributed level when added assay. A minimal incubation 30 min required detect lymphocytes. Effector could recruited for periods 90 >240 min, depending donor. conclude that: ( ) , IL-2-mediated due may lead an overestimate actual activity; b prolonged infusion after administration activated seems warranted order recruit maximal levels with