Tetrahydrobiopterin Role in human umbilical vein endothelial dysfunction in maternal supraphysiological hypercholesterolemia.
作者： Andrea Leiva , Bárbara Fuenzalida , Rocío Salsoso , Eric Barros , Fernando Toledo
DOI: 10.1016/J.BBADIS.2016.01.021
关键词: Endocrinology 、 Tetrahydrobiopterin 、 Biology 、 Sepiapterin 、 Electrical impedance myography 、 Internal medicine 、 Endothelial dysfunction 、 Umbilical vein 、 Endothelium 、 Nitric oxide synthase 、 Enos
摘要: Maternal physiological hypercholesterolemia (MPH) allows a proper foetal development; however, maternal supraphysiological (MSPH) associates with endothelial dysfunction and early development of atherosclerosis. MSPH courses reduced endothelium-dependent dilation the human umbilical vein due to nitric oxide synthase activity compared MPH. Whether modifies availability cofactor tetrahydrobiopterin is unknown. We investigated whether MSPH-associated lower vascular reactivity results from bioavailability tetrahydrobiopterin. Total cholesterol <7.2mmol/L was considered as (n=72 women) ≥7.2mmol/L (n=35 women). Umbilical veins rings were used for assays (wire myography), primary cultures cells (HUVECs) measure synthase, GTP cyclohydrolase 1, dihydrofolate reductase expression activity, well content. relaxation caused by calcitonine gene-related peptide, phenomenon partially improved incubation sepiapterin. HUVECs showed (l-citrulline synthesis l-arginine) without changes in its protein abundance, level MPH, reversed Expression 1 MSPH, expression. pathophysiological condition reducing leading NOS endothelium.
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