Changes in parathyroid hormone receptors during chondrocyte cytodifferentiation.
作者： H Baba , A Jikko , F Suzuki , K Nakashima , H Murakami
DOI: 10.1016/S0021-9258(17)32546-2
关键词: Receptor 、 Endocrinology 、 Parathyroid hormone receptor 、 Chondrocyte 、 Endochondral ossification 、 Alkaline phosphatase 、 Biology 、 Internal medicine 、 Proteoglycan 、 Parathyroid hormone 、 Cellular differentiation
摘要: The purpose of this study was to investigate the relationship between changes in parathyroid hormone (PTH) receptor levels and chondrocyte maturation during endochondral ossification. Chondrocytes were isolated from growth plate rabbit ribs maintained presence 10% serum mass cultures. Treatment with PTH-(1-84) a PTH-(1-34) fragment suppressed increases alkaline phosphatase activity type X collagen 1 alpha,25-dihydroxyvitamin D3 abolished 45Ca incorporation into mineral, all which occurred parallel untreated cultures hypertrophic (terminal) stage. These effects PTH observed at low concentrations (10(-10) 10(-9) M) within 24-48 h treatment. also increased [35S]sulfate newly synthesized proteoglycans. In contrast, middle carboxyl-terminal fragments tested had little effect on proteoglycan synthesis or terminal differentiation. binding 125I-PTH-(1-34) cells greater than that liver, skin, muscle, brain, kidney. When correlation stage examined, we found its 72-kDa resting proliferating chondrocytes, 10-fold matrix-forming thereafter decreased chondrocytes both vitro situ. Scatchard analysis revealed due number, not affinity, receptor. paralleled those findings suggest stage-dependent PTH/PTH-related peptide localize stimulation inhibition precocious hypertrophy zone plates.
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sci-hub.st 参考文章(38)
T M Schmid, H E Conrad, Metabolism of low molecular weight collagen by chondrocytes obtained from histologically distinct zones of the chick embryo tibiotarsus. Journal of Biological Chemistry. ,vol. 257, pp. 12451- 12457 ,(1982) , 10.1016/S0021-9258(18)33734-7
K D Schlüter, H Hellstern, E Wingender, H Mayer, The central part of parathyroid hormone stimulates thymidine incorporation of chondrocytes. Journal of Biological Chemistry. ,vol. 264, pp. 11087- 11092 ,(1989) , 10.1016/S0021-9258(18)60431-4
M Iwamoto, A Shimazu, K Nakashima, F Suzuki, Y Kato, Reduction of basic fibroblasts growth factor receptor is coupled with terminal differentiation of chondrocytes. Journal of Biological Chemistry. ,vol. 266, pp. 461- 467 ,(1991) , 10.1016/S0021-9258(18)52457-1
Otto A. Bessey, Oliver H. Lowry, Mary Jane Brock, A METHOD FOR THE RAPID DETERMINATION OF ALKALINE PHOSPHATASE WITH FIVE CUBIC MILLIMETERS OF SERUM Journal of Biological Chemistry. ,vol. 164, pp. 321- 329 ,(1946) , 10.1016/S0021-9258(18)43072-4
C Shigeno, Y Hiraki, D P Westerberg, J T Potts, G V Segre, Photoaffinity labeling of parathyroid hormone receptors in clonal rat osteosarcoma cells Journal of Biological Chemistry. ,vol. 263, pp. 3864- 3871 ,(1988) , 10.1016/S0021-9258(18)69005-2
Robert Robison, The Possible Significance of Hexosephosphoric Esters in Ossification Biochemical Journal. ,vol. 17, pp. 286- 293 ,(1923) , 10.1042/BJ0170286
M. Silbermann, R. Shurtz-Swirski, D. Lewinson, P. Shenzer, H. Mayer, In vitro response of neonatal condylar cartilage to simultaneous exposure to the parathyroid hormone fragments 1-34, 28-48, and 53-84 hPTH. Calcified Tissue International. ,vol. 48, pp. 260- 266 ,(1991) , 10.1007/BF02556377
H Juppner, A. Abou-Samra, M Freeman, X. Kong, E Schipani, J Richards, L. Kolakowski, J Hock, J. Potts, H. Kronenberg, al. et, A G protein-linked receptor for parathyroid hormone and parathyroid hormone-related peptide Science. ,vol. 254, pp. 1024- 1026 ,(1991) , 10.1126/SCIENCE.1658941
Gila Maori, Klaus Von Der Mark, Hari Reddi, Dick Heinegard, Ahnders Franzen, Michael Silbermann, Acceleration of Cartilage and Bone Differentiation on Collagenous Substrata Collagen and Related Research. ,vol. 7, pp. 351- 370 ,(1987) , 10.1016/S0174-173X(87)80028-6
Thorsten Kirsch, Klaus von der Mark, Ca2+binding properties of type X collagen FEBS Letters. ,vol. 294, pp. 149- 152 ,(1991) , 10.1016/0014-5793(91)81363-D