SEMA3B‐AS1‐inhibited osteogenic differentiation of human mesenchymal stem cells revealed by quantitative proteomics analysis
作者： Chen Zhang , Yun Zhu , Yugang Liu , Xiguang Zhang , Qiaoning Yue
DOI: 10.1002/JCP.26776
关键词: Isobaric tag for relative and absolute quantitation 、 Adult stem cell 、 Chemistry 、 Semaphorin 、 Mesenchymal stem cell 、 Cell biology 、 Focal adhesion 、 Actin cytoskeleton 、 Quantitative proteomics 、 Spliceosome
摘要: Human mesenchymal stem cells (hMSCs) are fibroblastoid multipotent adult with capacities of differentiation into osteoblasts and chondrocytes show great potential in new bone formation repair-related clinical settings, such as osteoporosis. Long noncoding RNAs (lncRNAs) have been demonstrated to play important roles various biological processes. Here, we report an antisense lncRNA SEMA3B-AS1 regulating hMSCs osteogenesis. is proximal a member the semaphorin family Sema3b. Overexpression using lentivirus system markedly inhibits proliferation meanwhile reduces osteogenic differentiation. Using comprehensive proteomic technique named isobaric tag for relative absolute quantitation, found that significantly alters process osteogenesis through downregulating expression proteins involved actin cytoskeleton, focal adhesion, extracellular matrix-receptor interaction, while increasing spliceosome. Collectively, find target controlling hMSCs.
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