DNA damage and methylation induced by glyphosate in human peripheral blood mononuclear cells (in vitro study)
作者： Marta Kwiatkowska , Edyta Reszka , Katarzyna Woźniak , Ewa Jabłońska , Jaromir Michałowicz
DOI: 10.1016/J.FCT.2017.03.051
关键词: DNA 、 Biology 、 Peripheral blood mononuclear cell 、 Molecular biology 、 Comet assay 、 DNA methylation 、 DNA damage 、 CDKN2A 、 Methylation 、 Glyphosate
摘要: Glyphosate is a very important herbicide that widely used in the agriculture, and thus exposure of humans to this substance its metabolites has been noted. The purpose study was assess DNA damage (determination single double strand-breaks by comet assay) as well evaluate methylation (global p16 (CDKN2A) p53 (TP53) promoter regions) human peripheral blood mononuclear cells (PBMCs) exposed glyphosate. PBMCs were incubated with compound studied at concentrations ranging from 0.1 10 mM for 24 h. shown glyphosate induced lesions, which effectively repaired. However, unable repair completely We also observed decrease global level 0.25 mM 0.5 mM increased methylation, while it did not induce statistically significant changes promoter. To sum up, we have first time (at high 0.5 10 mM) may leucocytes such cause cells.
sci-hub.se 参考文章(31)
R. Mesnage, N. Defarge, J. Spiroux de Vendômois, G.E. Séralini, Potential toxic effects of glyphosate and its commercial formulations below regulatory limits. Food and Chemical Toxicology. ,vol. 84, pp. 133- 153 ,(2015) , 10.1016/J.FCT.2015.08.012
Minoru Toyota, Eiichiro Yamamoto, DNA methylation changes in cancer. Progress in Molecular Biology and Translational Science. ,vol. 101, pp. 447- 457 ,(2011) , 10.1016/B978-0-12-387685-0.00014-7
Marta Kulis, Manel Esteller, DNA methylation and cancer. Advances in Genetics. ,vol. 70, pp. 27- 56 ,(2010) , 10.1016/B978-0-12-380866-0.60002-2
Danuta Pieniążek, Bożena Bukowska, Wirgiliusz Duda, Comparison of the effect of Roundup Ultra 360 SL pesticide and its active compound glyphosate on human erythrocytes Pesticide Biochemistry and Physiology. ,vol. 79, pp. 58- 63 ,(2004) , 10.1016/J.PESTBP.2004.03.003
K. Zouaoui, S. Dulaurent, J.M. Gaulier, C. Moesch, G. Lachâtre, Determination of glyphosate and AMPA in blood and urine from humans: about 13 cases of acute intoxication. Forensic Science International. ,vol. 226, ,(2013) , 10.1016/J.FORSCIINT.2012.12.010
Xiao Zhang, Andrew D. Wallace, Pan Du, Simon Lin, Andrea A. Baccarelli, Hongmei Jiang, Nadereh Jafari, Yinan Zheng, Hehuang Xie, Marcelo Bento Soares, Warren A. Kibbe, Lifang Hou, Genome-wide study of DNA methylation alterations in response to diazinon exposure in vitro Environmental Toxicology and Pharmacology. ,vol. 34, pp. 959- 968 ,(2012) , 10.1016/J.ETAP.2012.07.012
Verena J. Koller, Maria Fürhacker, Armen Nersesyan, Miroslav Mišík, Maria Eisenbauer, Siegfried Knasmueller, Cytotoxic and DNA-damaging properties of glyphosate and Roundup in human-derived buccal epithelial cells Archives of Toxicology. ,vol. 86, pp. 805- 813 ,(2012) , 10.1007/S00204-012-0804-8
Marco Aurelio Romano, Renata Marino Romano, Luciana Dalazen Santos, Patricia Wisniewski, Daniele Antonelo Campos, Paula Bargi de Souza, Priscila Viau, Maria Martha Bernardi, Maria Tereza Nunes, Claudio Alvarenga de Oliveira, Glyphosate impairs male offspring reproductive development by disrupting gonadotropin expression Archives of Toxicology. ,vol. 86, pp. 663- 673 ,(2012) , 10.1007/S00204-011-0788-9
Janusz Błasiak, Joanna Kowalik, A comparison of the in vitro genotoxicity of tri- and hexavalent chromium Mutation Research/Genetic Toxicology and Environmental Mutagenesis. ,vol. 469, pp. 135- 145 ,(2000) , 10.1016/S1383-5718(00)00065-6
Eliane Dallegrave, Fabiana D. Mantese, Rosemari T. Oliveira, Anderson J. M. Andrade, Paulo R. Dalsenter, Augusto Langeloh, Pre- and postnatal toxicity of the commercial glyphosate formulation in Wistar rats Archives of Toxicology. ,vol. 81, pp. 665- 673 ,(2007) , 10.1007/S00204-006-0170-5