Sex determination of superorder Neognathae (class Aves) by molecular genetics methods
作者： Anna Trakovická , Martina Miluchová , Michal Gábor , Eva Radosova , Cyril Hrnčár
DOI:
关键词: Superorder 、 genomic DNA 、 Genetics 、 Genome 、 Restriction site 、 Neognathae 、 Molecular genetics 、 Molecular biology 、 Biology 、 Restriction enzyme 、 Gene
摘要: The aim of this study was optimization molecular genetic method for sex determination superorder Neognathae from class Aves. Molecular-genetic methods based on the amplification a chromo-helicase DNA binding 1 (CHD) gene region, which is located in both chromozomes Z and W. Genomic isolated whole blood and feathers by using commercial column kit QIAamp Mini kit. intron regions CHDW CHDZ genes were amplified specific primers P2 P8. PCR used two differences between genes. One them restriction site endonuclease Hae III only second lenght polymorphism where males detected one band females bands 3 % agarose gel. These molecular-genetics successfully 36 species .
cabdirect.org参考文章(12)
A. Dubiec, M. Zagalska-Neubauer, Molecular techniques for sex identification in birds Biological Letters. ,vol. 43, ,(2006)
Milos Vucicevic, Marija Stevanov-Pavlovic, Jevrosima Stevanovic, Jasna Bosnjak, Bojan Gajic, Nevenka Aleksic, Zoran Stanimirovic, Sex determination in 58 bird species and evaluation of CHD gene as a universal molecular marker in bird sexing. Zoo Biology. ,vol. 32, pp. 269- 276 ,(2013) , 10.1002/ZOO.21010
Richard Griffiths, Bela Tiwari, Sex of the last wild Spix's macaw Nature. ,vol. 375, pp. 454- 454 ,(1995) , 10.1038/375454A0
Paola Sacchi, Dominga Soglia, Sandra Maione, Giuseppe Meneguz, Massimo Campora, Roberto Rasero, A non-invasive test for sex identification in Short-toed Eagle (Circaetus gallicus) Molecular and Cellular Probes. ,vol. 18, pp. 193- 196 ,(2004) , 10.1016/J.MCP.2004.01.002
Sex identification in birds using two CHD genes Proceedings of The Royal Society B: Biological Sciences. ,vol. 263, pp. 1251- 1256 ,(1996) , 10.1098/RSPB.1996.0184
M. Clinton, A rapid protocol for sexing chick embryos (Gallus g. domesticus) Animal Genetics. ,vol. 25, pp. 361- 362 ,(2009) , 10.1111/J.1365-2052.1994.TB00374.X
RICHARD GRIFFITHS, MIKE C. DOUBLE, KATE ORR, ROBERT J. G. DAWSON, A DNA test to sex most birds Molecular Ecology. ,vol. 7, pp. 1071- 1075 ,(1998) , 10.1046/J.1365-294X.1998.00389.X
Anna-Karin Fridolfsson, Hans Ellegren, A Simple and Universal Method for Molecular Sexing of Non-Ratite Birds Journal of Avian Biology. ,vol. 30, pp. 116- 121 ,(1999) , 10.2307/3677252
DAIZABURO SHIZUKA, BRUCE E. LYON, Improving the reliability of molecular sexing of birds using a W-specific marker. Molecular Ecology Resources. ,vol. 8, pp. 1249- 1253 ,(2008) , 10.1111/J.1755-0998.2008.02342.X
First Gene on the Avian W Chromosome (CHD) Provides a Tag for Universal Sexing of Non-Ratite Birds Proceedings of The Royal Society B: Biological Sciences. ,vol. 263, pp. 1635- 1641 ,(1996) , 10.1098/RSPB.1996.0239