MicroRNA signature associated with osteogenic lineage commitment

摘要: Cell-based approaches offer a potential therapeutic strategy for appropriate bone manufacturing. Capable of differentiating into multiple cell types especially osteoblasts spontaneously, unrestricted somatic stem (USSC) seems to be suitable candidate. Recent studies have shown the involvement microRNAs in several biological processes. miRNA microarray profiling was applied order identify osteo-specific signature. Prior this analysis, osteogenic commitment evaluated by measuring ALPase activity, biomineralization, specific staining and evaluation some main marker genes. To support our findings, various silico explorations (for both putative targets signaling pathways) empirical analyses (miRNA transfections followed qPCR indicators activity measurement) were carried out. The function GSK-3b inhibitor also studied investigate role WNT osteogenesis. Transient modulation osteo-miRs (such as mir-199b, 1274a, 30b) with common BMPR, TCFs, SMADs) mediators pathways including cell-cell interactions, TGF-beta pathways, suggests mechanism rapid induction osteogenesis an anti-miRNA therapy. results research identified signature which regulates USSC. conclude, study reveals more details about allocation USSCs lineage through modulatory effect miRNAs on required creating tissue-specific phenotype may aid future clinical interventions.