Cytokine and antibody subclass responses in the intestinal lymph of sheep during repeated experimental infections with the nematode parasite Trichostrongylus colubriformis.

摘要: The expression of interleukin (IL)-4, IL-5, IL-10, IL-13, TNF-alpha and IFN-gamma genes, parasite-specific IgM, IgG1, IgG2, IgA total IgE levels, were monitored daily in intestinal lymph sheep infected repeatedly with the nematode parasite Trichostrongylus colubriformis. Host genotype had a significant influence on IL-13 gene activity, resistant-line (R) consistently expressing higher levels mRNA than susceptible-line (S) sheep. Mean IL-4 did not differ significantly between first second challenge. Field-primed R S as well field-primed naive lower mean IL-5 respectively, during when compared to primary Genes for transiently strongly up-regulated after infection, particularly animals previous exposure nematodes. also up-regulated, but lesser extent more typically Naive both genotypes produced relatively little antibody response A challenge resulted large increases lymphatic all sub-classes which adult antigen-specific larval IgG1. In line sheep, larval-specific IgG2 appeared delayed animals. high IgG1 prior experimental infection these change afterwards. These results demonstrate that infections, micro-environment is skewed towards Th2 cytokine dominance, although continues. This accompanied by nematode-specific IgA, plasma.