Effects of a time-varying strong magnetic field on transient increase in Ca2+ release induced by cytosolic Ca2+ in cultured pheochromocytoma cells

作者: Toshitaka Ikehara , Hisao Yamaguchi , Keiko Hosokawa , Hitoshi Houchi , Ki Ho Park

DOI: 10.1016/J.BBAGEN.2005.03.008

关键词: Inositol trisphosphate receptorBiophysicsEndoplasmic reticulumIntracellularCell membraneATP hydrolysisBiochemistryChemistryRyanodine receptorCyclic ADP-riboseCytosol

摘要: Abstract Exposure of pheochromocytoma (PC 12) cells to a time-varying 1.51 T magnetic field inhibited an increase in the intracellular Ca2+ concentration ([Ca2+]i) induced by addition caffeine Ca2+-free medium. This inhibition occurred after 15-min exposure and was maintained for at least 2 h. [Ca2+]i sharply increased loaded with cyclic ADP-ribose, 2-h significantly suppressed increase. Addition ATP transient release mediated IP3 receptor, this strongly exposure. Results indicated that both ryanodine receptors PC 12 cells. However, thapsigargin-induced influx (capacitative entry) across cell membrane unaffected. The content normal level during exposure, suggesting hydrolysis unchanged. Therefore, Mg2+ which is known be released inhibit may not relate exposure-caused inhibition. Eddy currents culture medium appear change properties indirectly from endoplasmic reticulum other stores

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