Histochemistry of Lysosomes

摘要: Publisher Summary This chapter focuses on the histochemistry of lysosomes. Lysosomes are defined originally as a biochemical entity. Consequently, criteria form basis for histochemical identification these particles. Ideally, prime which appear necessary demonstration lysosome (1) presence one or more acid hydrolases, known to be associated with lysosomes, at particulate sites; (2) such sites should possess single limiting membrane; (3) activation by other methods discussed particular hydrolases studied ; and ( 4 ) reaction particles hematein test, periodic acid-Schiff reaction, vital dyes. The studies have contributed extending concept from “a bag hydrolytic enzymes” in has assumed physiological importance. findings points strongly role intracellular digestion. This, fact, may prove essence all lysosomal functions, while there changes behavior pattern under wide range cellular activities, mitosis, stress, virus infection, underlying function lysosomes is still likely discusses enzymic nonenzymic demonstrating identlficatlon various tissues, evidence like