An overview on functional receptor autoradiography using [35S]GTPγS

摘要: Abstract [35S]GTPγS binding autoradiography is a novel method to study the distribution and function of neurotransmitter receptors in tissue sections. This technique unifies advantages receptor-autoradiography binding, providing anatomical functional information at same time. Due these two main features, it can also be called ‘functional autoradiography’. has long been used first step intracellular signaling pathway, but until mid 1990s only performed on cell membrane extracts. Functional evolved from this biochemical assay ligand autoradiography, based increase guanine nucleotide exchange G-proteins upon agonist stimulation. With technique, activation G-protein-coupled detected, and, time, location activated visualized. Thus those presumably active are visualized that involved signal transduction. In past 5 years become more frequently neuroscience, adapted several different species, including human brain. describe receptors. Some inferences their coupling efficiency drawn. Besides localization sites, provides action receptor: agonists, antagonists, inverse agonists clearly distinguished. Moreover, successfully combined with other vitro assays, like receptor situ hybridization histochemistry, or even electrophysiological experiments. review presents an overview history development technique. Its limitations summarized, together few basic technical questions. A number experiments so far, some possible applications for future, reviewed.