作者: Joanne E. Hughes , Dennis L. Welker
DOI: 10.1007/978-1-4615-4707-5_1
关键词: Dictyostelium 、 Computational biology 、 Rolling circle replication 、 Biology 、 Plasmid maintenance 、 Plasmid 、 Organism 、 Extrachromosomal DNA
摘要: Decades of studying plasmids in bacterial systems have led to their characterization as autonomously replicating, extrachromosomal DNA molecules carrying no elements essential the viability organism. The information obtained through examination provided foundation on which much progress genetic engineering and molecular biology rests (1, 2, 3). Knowledge plasmid - copy number control, compatibility, maintenance strategies, replication requirements enabled design vectors with specific characteristics allowing otherwise impractical experiments.