A possible role of the key enzymes of the glyoxylate and gluconeogenesis pathways for fruit-body formation of the wood-rotting basidiomycete Flammulina velutipes

摘要: Biochemical roles of the representative enzymes involved in carbon metabolism glucose were investigated relation to fruit-body formation basidiomycete Flammulina velutipes. Changes specific activities tricarboxylic acid (TCA) cycle and glyoxylate (GLOX) gluconeogenesis pathways measured at different stages development fungus. The enzyme malate synthase (MS) fructose bisphosphatase (FBP) as key for GLOX-gluconeogenesis increased mycelia during formation. isocitrate dehydrogenase (IDH) TCA NADP-linked glutamate (GLTDH (NADP)) synthesis more markedly. Moreover, mycelial mat cultures producing fruit bodies yielded greater lyase (ICL), MS, FBP, IDH than that did not produce bodies. These results suggest well have a strong correlation with F.