Glioma inhibition by HGF/NK2, an antagonist of scatter factor/hepatocyte growth factor.

摘要: Abstract Strategies that antagonize growth factor signaling are attractive candidates for the biological therapy of brain tumors. HGF/NK2 is a secreted truncated splicing variant and potential antagonist scatter factor/hepatocyte (SF/HGF), multifunctional cytokine involved in malignant progression solid tumors including glioblastoma. U87 human glioma cells express an autocrine SF/HGF stimulatory loop were transfected with cDNA clonal cell lines secrete high levels protein (U87-NK2) isolated. The effects gene transfer on phenotype examined. had no effect 2-dimensional anchorage-dependent growth. In contrast, U87-NK2 ∼20-fold less clonogenic soft agar ∼4-fold migratory than control-transfected lines. Intracranial tumor xenografts derived from grew much slower controls. ∼50-fold smaller controls at 21 days post-implantation resulted trend toward diminished tumorigenicity. This report shows predominant transgenic overexpression by stimulation to inhibit their phenotype.