Molecular analysis of the dmpM gene encoding an O-demethyl puromycin O-methyltransferase from Streptomyces alboniger.
作者： Rosa Ana Lacalle , Diego Ruiz , Antonio Jiménez
DOI: 10.1016/0378-1119(91)90588-3
关键词: Reading frame 、 Puromycin 、 Start codon 、 Nucleic acid sequence 、 Peptide sequence 、 Stop codon 、 Molecular biology 、 Biology 、 Streptomyces alboniger 、 Open reading frame
摘要: The nucleotide (nt) sequence of a 1332-bp fragment Streptomyces alboniger DNA containing the gene (dmpM), which encodes an O-demethyl puromycin O-methyltransferase (DMPM), has been determined. dmpM contains 1131-nt open reading frame polypeptide Mr 40,303; this is consistent with 44 +/- 2.5- and 160-kDa sizes DMPM monomer its native form, respectively. ATG start codon 50 bp downstream from coding (pac), determines N-acetyltransferase. S1 mapping experiments indicate that pac are transcribed on single transcript, ends at least 500 nt stop codon. deduced amino acid shows significant similarities to those hydroxyindole O-methyltransferase, involved in biosynthesis melatonin by bovine pineal glands [Ishida et al., J. Biol. Chem. 262 (1987) 2895-2899], hydroxyneurosporene methyltransferase, carotenoid purple nonsulfur bacterium, Rhodobacter capsulatus [Armstrong Mol. Gen. Genet. 216 (1989) 254-268] two O-methyltransferases tetracenomycin pathway glaucescens.
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