Overexpression of tomato mitogen-activated protein kinase SlMPK3 in tobacco increases tolerance to low temperature stress

摘要: Mitogen-activated protein kinase (MAPK) cascades are important intracellular signaling events that mediate signal transduction associated with plant responses to environmental stresses. In this study, tomato plants were subjected treatments of low (4 °C) and high-temperature (42 °C) exogenous application methyl jasmonate, salicylic acid hydrogen peroxide. Results show leaves from treated contained an elevated level the MAPK gene (SlMPK3). Transient expression assay onion epidermal cells using pBI121-GFP constructs indicate SlMPK3 was predominantly localized in nuclei. transgenic tobacco overexpressing SlMPK3, less reactive oxygen species (ROS), more superoxide dismutase, peroxidase, catalase, ascorbate peroxidase activities, higher proline soluble sugar contents, transcript stress-responsive genes. Seeds derived those had a significant germination rate (P < 0.05) under chilling stress (12 °C). The seedlings also developed larger root system same temperature treatment conditions. Taken together, these data demonstrate acts as positive regulator response by regulating ROS homeostasis through activation cellular antioxidant systems modulating transcription stress-associated