Ribosyl Geometry in the Transition State of Streptococcus pneumoniae Methylthioadenosine Nucleosidase from the 3'-2H Kinetic Isotope Effect
作者： Minkui Luo , Vern L. Schramm
DOI: 10.1021/JA804578M
关键词: Catalysis 、 Chemistry 、 Transition (genetics) 、 Organic chemistry 、 Streptococcus pneumoniae 、 Methylthioadenosine nucleosidase 、 Stereochemistry 、 Kinetic isotope effect
摘要: Synthesis of [3′-2H]-labeled 5′-methylthioadenosine (MTA) derivatives permitted measurement the [3′-2H] KIE reaction catalyzed by Streptococcus pneumoniae methylthioadenosine nucleosidase (spMTAN). The key revealed partial 3′-OH polarization and H3′-endo→exo ribosyl configuration at spMTAN transition state. A new understanding state stabilization spMTAN-catalyzed hydrolysis is uncovered in structural features
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