Engineering craniofacial scaffolds

摘要: Structured Abstract: Authors –  Hollister SJ, Lin CY, Saito E, Schek RD, Taboas JM, Williams Partee B, Flanagan CL, Diggs A, Wilke EN, Van Lenthe GH, Muller R, Wirtz T, Das S, Feinberg SE, Krebsbach PH Objective To develop an integrated approach for engineering craniofacial scaffolds and to demonstrate that these engineered would have mechanical properties in the range of tissue support bone regeneration reconstruction. Experimental Variable Scaffold architecture designed achieve desired elasticity permeability. external shape match anatomy. Outcome Measure Final fabricated biomaterial scaffolds. Compressive modulus strength. Bone as measured by micro-CT scanning, testing histology. Setting Departments Biomedical Engineering, Oral/Maxillofacial Surgery, Oral Medicine, Pathology Oncology at University Michigan. Results Results showed design/fabrication could create with porous anatomy. These be from a wide biomaterials, including titanium, degradable polymers, calcium phosphate ceramics. Mechanical tests had compressive ranging 50 2900 MPa strength 2 over 56 MPa, within human trabecular bone. In vivo they via delivery BMP-7 transduced gingival fibroblasts mouse model. Designed hydroxyapatite pore diameters 400 1200 microns were implanted minipig mandibular defects 6 18 weeks. substantial ingrowth (between 40 50% 6 weeks, between 70 80% 18 weeks) all scaffolds, no significant difference based on diameter. Conclusion Integrated image-based design solid free-form fabrication can attain permeability while fitting any 3D defect. The manufactured ceramics titanium. supported sizes 300 1200 microns. results suggest are clinically applicable complex reconstruction.