Optimisation of a simultaneous separation of sulphonamides, dihydrofolate reductase inhibitors and β-lactam antibiotics by capillary electrophoresis

摘要: Abstract There are many high-performance liquid chromatographic assays for the determination of sulphonamides, dihydrofolate reductase inhibitors and β-lactam antibiotics individually in food products. Recently, some capillary electrophoresis (CE) these drugs have appeared. Sulphonamides plus readily separated by zone (CZE) micellar electrokinetic chromatography (MECC) can separate β-lactams. However, if CE is to prove its value over HPLC techniques practice then intrinsic high resolution must be exploited fully. A combination a sulphonamide-dihydrofolate separation CZE with MECC assay β-lactams would advantageous screening products veterinary drug residues, saving both time effort. The analysis all three classes drugs, single run, was tested using our established conditions, but this gave only partial separation, very crowded region. Box-Behnken factorial design used optimise separation. It carried out parallel “traditional trial error procedure”, based on knowledge individual separations. Both methods arrived at remarkably similar set conditions. final produced 25 peaks, within which, were 30 compounds, taking min.