Effect of phenylalanine and α-methylphenylalanine on in vitro incorporation of 32P into cytoskeletal cerebral proteins
作者： M DEFREITAS , A DEMATTOS , M CAMARGO , C WANNMACHER , R PESSOAPUREUR
DOI: 10.1016/0197-0186(94)00142-H
关键词: Hyperphenylalaninemia 、 Cerebral cortex 、 In vitro 、 Phosphorylation 、 Cytoskeleton 、 Neurofilament 、 Biochemistry 、 Biology 、 Tubulin 、 Phenylalanine
摘要: Abstract We studied the effects of L-phenylalanine and a-methylphenylalanine on 32P in vitro incorporation into cytoskeletal proteins from cerebral cortex 17-day-old rats. Slices were incubated absence or presence increasing concentrations l -phenylalanine, α-methylphenylalanine -phenylalanine plus for 1 h. The fraction obtained slices was same drugs measured. Addition did not change proteins, but phenylalanine decreased phosphorylation β tubulin. Furthermore, addition both 160 kDa neurofilaments α-tubulin.
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