Failure of apoptosis-inducing factor to act as neuroglobin reductase

摘要: Neuroglobin (Ngb) is a hexacoordinate globin expressed in the nervous system of vertebrates, where it protects neurons against hypoxia. Ferrous Ngb has been proposed to favor cell survival by scavenging NO and/or reducing cytochrome c released into cytosol during hypoxic stress. Both catalytic functions require an as yet unidentified Ngb-reductase activity. Such activity was detected both tissue homogenates human brain and liver Escherichia coli extracts. Since NADH:flavorubredoxin oxidoreductase from E. coli, that shown reduce ferric Ngb, shares sequence similarity with apoptosis-inducing factor (AIF), AIF us candidate reductase. In this study, we tested hypothesis show recombinant negligible hence incompatible such physiological function.