Combined use of [TBA][L-ASP] and hydroxypropyl-β-cyclodextrin as selectors for separation of Cinchona alkaloids by capillary electrophoresis

摘要: In this paper, a new capillary electrophoresis (CE) separation and detection method was developed for the chiral of four major Cinchona alkaloids (quinine/quinidine cinchonine/cinchonidine) using hydroxypropyl-β-cyclodextrin (HP-β-CD) ionic liquid ([TBA][L-ASP]) as selectors. Separation parameters such buffer concentrations, pH, HP-β-CD temperature, voltage were investigated. After optimization conditions, baseline three analytes (cinchonidine, quinine, cinchonine) achieved in fewer than 7 min ammonium acetate background electrolyte (pH 5.0) with addition concentration 40 mM [TBA][L-ASP] 14 mM, while cinchonine quinidine not obtained. Therefore, first-order derivative electropherogram applied resolving overlapping peaks. Regression equations revealed good linear relationship between peak areas electropherograms concentrations two diastereomer pairs. The results only indicated that effective determination low content component those fully separated from adjacent ones, but also showed appeared to be very promising selector CE.