Molar absorption coefficients for the reduced Ellman reagent: reassessment.

作者: Peter Eyer , Franz Worek , Daniela Kiderlen , Goran Sinko , Anita Stuglin

DOI: 10.1016/S0003-2697(02)00506-7

关键词: ReagentDithionitrobenzoic AcidThermochromismAnalytical chemistryMolar absorptivityAbsorption (chemistry)AbsorbanceChromogenicChemistryMolar

摘要: … The TNB molar absorption coefficient, 13.6×10 3 M −1 cm −1 , … Our recommended molar absorption coefficients at 412 nm are … Molar absorption coefficients for other temperatures and …

参考文章(12)
Offutt Dm, VanGoethem D, Mejia Mb, Thyssen Jh, Christenson Wr, Landes Mm, Sangha Gk, Dass Pd, Stuart Bp, Comparative kinetic analysis of cholinesterase methods in rat and human erythrocytes and plasma. Veterinary and Human Toxicology. ,vol. 39, pp. 11- 17 ,(1997)
T A Walmsley, M H Abernethy, H P Fitzgerald, Effect of daylight on the reaction of thiols with Ellman's reagent, 5,5'-dithiobis(2-nitrobenzoic acid). Clinical Chemistry. ,vol. 33, pp. 1928- 1931 ,(1987) , 10.1093/CLINCHEM/33.10.1928
Franz Worek, Ulrike Mast, Daniela Kiderlen, Christine Diepold, Peter Eyer, Improved determination of acetylcholinesterase activity in human whole blood. Clinica Chimica Acta. ,vol. 288, pp. 73- 90 ,(1999) , 10.1016/S0009-8981(99)00144-8
George L. Ellman, A colorimetric method for determining low concentrations of mercaptans Archives of Biochemistry and Biophysics. ,vol. 74, pp. 443- 450 ,(1958) , 10.1016/0003-9861(58)90014-6
George L. Ellman, K.Diane Courtney, Valentino Andres, Robert M. Featherstone, A new and rapid colorimetric determination of acetylcholinesterase activity Biochemical Pharmacology. ,vol. 7, pp. 88- 95 ,(1961) , 10.1016/0006-2952(61)90145-9
George L. Ellman, Tissue sulfhydryl groups Archives of Biochemistry and Biophysics. ,vol. 82, pp. 70- 77 ,(1959) , 10.1016/0003-9861(59)90090-6
Peter W. Riddles, Robert L. Blakeley, Burt Zerner, Ellman's reagent: 5,5′-dithiobis(2-nitrobenzoic acid)—a reexamination Analytical Biochemistry. ,vol. 94, pp. 75- 81 ,(1979) , 10.1016/0003-2697(79)90792-9