Optical mapping discerns genome wide DNA methylation profiles
作者： Gene E Ananiev , Steve Goldstein , Rod Runnheim , Dan K Forrest , Shiguo Zhou
关键词: Genomics 、 RNA-Directed DNA Methylation 、 Methylation 、 DNA methylation 、 Epigenomics 、 Biology 、 Genome 、 Epigenetics 、 Genetics 、 Illumina Methylation Assay
摘要: Methylation of CpG dinucleotides is a fundamental mechanism epigenetic regulation in eukaryotic genomes. Development methods for rapid genome wide methylation profiling will greatly facilitate both hypothesis and discovery driven research the field epigenetics. In this regard, single molecule approach to offers several unique advantages that include elimination chemical DNA modification steps PCR amplification. A presented discernment profiles, based on optical mapping. We report results from series pilot studies demonstrating capabilities mapping as platform whole Optical was used discern profile an engineered wild type Escherichia coli. Furthermore, status selected loci within human embryonic stem cells profiled using The effectively detects patterns. Due detection, significant over other technologies. This advantage stems obviation steps, such bisulfite treatment, ability assay repeat dense regions mammalian genomes inaccessible techniques array-hybridization
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