Stem cells of the apical papilla regulate trigeminal neurite outgrowth and targeting through a BDNF-dependent mechanism.

摘要: Regenerative endodontic procedures have become a valuable alternative for the treatment of immature teeth with pulp necrosis. In addition to resolution periradicular pathosis and promotion continued root development, positive vitality testing has been observed in some regenerative clinical cases. Importantly, response electric stimulation regenerated tissue requires targeting axons into previously empty canal space. However, mechanism by which this process occurs is largely unknown. Since stem cells apical papilla (SCAP) proposed populate following procedures, we hypothesized that SCAP regulate neurite outgrowth axonal targeting. To test hypothesis, established primary co-cultures human rat trigeminal neurons, performed assays using ELISA confocal microscopy determine effect increasing concentration on total addition, evaluated whether evoked vivo matrigel subcutaneous implant assay. Data were analyzed ANOVA Bonferroni's post hoc test, significance was set at p<0.05. The results demonstrated release soluble factor regulates from cultured neurons. Next, completely abolished pretreatment neutralizing antibody brain-derived neurotrophic (BDNF), but not antibodies other neurotrophins. Further, BDNF concentration-dependent manner as detected ELISA, trigger directed both vitro microfluidic experiments, respectively. Collectively, these suggest may be responsible chemical signal driving target via BDNF-dependent mechanism.