Quantitation of targets for PCR by use of limiting dilution.
作者： M J Brisco , A A Morley , P J Sykes , E Hughes , J Condon
DOI:
关键词: Biology 、 Polymerase chain reaction 、 DNA 、 Genome 、 Quantitative analysis (chemistry) 、 Gene rearrangement 、 Molecular biology 、 Gene 、 Immunoglobulin heavy chain 、 clone (Java method)
摘要: … In this paper we illustrate the use of limiting dilution analysis to quantitate a target for the PCR… : optimization of the PCR to detect one or a few DNA targets; the effect of excess competing …
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