Computer-assisted assignment of functional domains in the nonstructural polyprotein of hepatitis E virus: delineation of an additional group of positive-strand RNA plant and animal viruses
作者： E. V. Koonin , A. E. Gorbalenya , M. A. Purdy , M. N. Rozanov , G. R. Reyes
关键词: Beet necrotic yellow vein virus 、 RNA polymerase 、 Genetics 、 RNA Helicase A 、 Plant virus 、 Polymerase 、 RNA 、 RNA-dependent RNA polymerase 、 Virus 、 Virology 、 Biology
摘要: Computer-assisted comparison of the nonstructural polyprotein hepatitis E virus (HEV) with proteins other positive-strand RNA viruses allowed identification following putative functional domains: (i) RNA-dependent polymerase, (ii) helicase, (iii) methyltransferase, (iv) a domain unknown function ("X" domain) flanking papain-like protease domains in polyproteins animal viruses, and (v) cysteine distantly related to rubella (RubV). Comparative analysis polymerase helicase sequences belonging so-called "alpha-like" supergroup revealed grouping between HEV, RubV, beet necrotic yellow vein (BNYVV), plant furovirus. Two additional have been identified: one showed significant conservation BNYVV, specifically HEV RubV. The large BNYVV retained similar organization, exceptions relocation as compared RubV absence X BNYVV. These observations show that encompass partially conserved arrays distinctive domains, suggesting these constitute distinct monophyletic group within alpha-like viruses.
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