Cystic fibrosis epithelial cells are primed for apoptosis as a result of increased Fas (CD95)
作者： Qiwei Chen , Sudha Priya Soundara Pandi , Lauren Kerrigan , Noel G. McElvaney , Catherine M. Greene
DOI: 10.1016/J.JCF.2018.01.010
关键词: Cancer research 、 Cystic fibrosis 、 Apoptosis 、 Basal (phylogenetics) 、 Fas receptor 、 Cystic fibrosis transmembrane conductance regulator 、 Receptor 、 Medicine 、 Bronchial Epithelial Cell 、 Extrinsic apoptosis
摘要: Abstract Background Previous work suggests that apoptosis is dysfunctional in cystic fibrosis (CF) airways with conflicting results. We evaluated the relationship between transmembrane conductance regulator (CFTR) and CF airway epithelial cells. Methods Apoptosis associated caspase activity were analysed non-CF tracheal bronchial cell lines. Results Basal levels of caspase-3 caspase-8 significantly increased cells compared to controls, suggesting involvement extrinsic signalling, which mediated by activation death receptors, such as Fas (CD95). Increased observed brushings from patients controls. Neutralisation inhibited untreated In addition, control Conclusions Overall, these results suggest are more sensitive via subsequent receptor pathway, may be CFTR.
cysticfibrosisjournal.com参考文章(30)
Irene K. Oglesby, Isabella M. Bray, Sanjay H. Chotirmall, Raymond L. Stallings, Shane J. O’Neill, Noel G. McElvaney, Catherine M. Greene, miR-126 Is Downregulated in Cystic Fibrosis Airway Epithelial Cells and Regulates TOM1 Expression Journal of Immunology. ,vol. 184, pp. 1702- 1709 ,(2010) , 10.4049/JIMMUNOL.0902669
Mathilde Rottner, Corinne Kunzelmann, Martine Mergey, Jean‐Marie Freyssinet, Maria Carmen Martínez, Exaggerated apoptosis and NF-kappaB activation in pancreatic and tracheal cystic fibrosis cells. The FASEB Journal. ,vol. 21, pp. 2939- 2948 ,(2007) , 10.1096/FJ.06-7614COM
Irina Petrache, Viswanathan Natarajan, Lijie Zhen, Terry R Medler, Amy T Richter, Chung Cho, Walter C Hubbard, Evgeny V Berdyshev, Rubin M Tuder, Ceramide upregulation causes pulmonary cell apoptosis and emphysema-like disease in mice. Nature Medicine. ,vol. 11, pp. 491- 498 ,(2005) , 10.1038/NM1238
Ebru Yalçin, Beril Talim, Uğur Özçelik, Deniz Doğru, Nazan Çobanoğlu, Sevgi Pekcan, Nural Kiper, Does defective apoptosis play a role in cystic fibrosis lung disease Archives of Medical Research. ,vol. 40, pp. 561- 564 ,(2009) , 10.1016/J.ARCMED.2009.07.005
Henning R. Stennicke, Juliane M. Jürgensmeier, Hwain Shin, Quinn Deveraux, Beni B. Wolf, Xiaohe Yang, Qiao Zhou, H. Michael Ellerby, Lisa M. Ellerby, Dale Bredesen, Douglas R. Green, John C. Reed, Christopher J. Froelich, Guy S. Salvesen, Pro-caspase-3 Is a Major Physiologic Target of Caspase-8 Journal of Biological Chemistry. ,vol. 273, pp. 27084- 27090 ,(1998) , 10.1074/JBC.273.42.27084
Malgorzata Mrugacz, Fas Expression in Conjunctival Epithelial Cells of Patients With Cystic Fibrosis Journal of Interferon and Cytokine Research. ,vol. 29, pp. 735- 740 ,(2009) , 10.1089/JIR.2008.0111
Mark P. Rogan, David A. Stoltz, Douglas B. Hornick, Cystic Fibrosis Transmembrane Conductance Regulator Intracellular Processing, Trafficking, and Opportunities for Mutation-Specific Treatment Chest. ,vol. 139, pp. 1480- 1490 ,(2011) , 10.1378/CHEST.10-2077
Manish Bodas, Taehong Min, Neeraj Vij, Critical role of CFTR-dependent lipid rafts in cigarette smoke-induced lung epithelial injury. American Journal of Physiology-lung Cellular and Molecular Physiology. ,vol. 300, ,(2011) , 10.1152/AJPLUNG.00408.2010
V Kumar, T Becker, S Jansen, A van Barneveld, K Boztug, S Wölfl, B Tümmler, F Stanke, Expression levels of FAS are regulated through an evolutionary conserved element in intron 2, which modulates cystic fibrosis disease severity. Genes and Immunity. ,vol. 9, pp. 689- 696 ,(2008) , 10.1038/GENE.2008.63
Naoki Hagimoto, Kazuyoshi Kuwano, Hiroyuki Miyazaki, Ritsuko Kunitake, Masaki Fujita, Masayuki Kawasaki, Yumi Kaneko, Nobuyuki Hara, Induction of Apoptosis and Pulmonary Fibrosis in Mice in Response to Ligation of Fas Antigen American Journal of Respiratory Cell and Molecular Biology. ,vol. 17, pp. 272- 278 ,(1997) , 10.1165/AJRCMB.17.3.2893