Genetic Engineering of Mice to Investigate Uterine Function in Early Pregnancy

作者: Margeaux Wetendorf , Francesco J. DeMayo

DOI: 10.1016/B978-0-12-394445-0.00027-8

关键词: DecidualizationImmunologyBiologyTransgeneCell biologyGeneRecombinaseConditional gene knockoutPhenotypeGermlineUterus

摘要: Chapter Summary The generation and use of mouse models has been instrumental for today’s understanding uterine function fertility. Early including transgenic germline ablation mice often serendipitously demonstrated importance to biology as they displayed a phenotype. These models, although informative, exhibited limitations due their inability target the genetic modification directly uterus. Through recombination-inducing enzymes known recombinases palindromic recognition sequences, conditional knockout was generated. Conditional have developed with in vivo gene targeted uterus or specific compartment. overexpressing are recently advanced, innovative that complement modern techniques further aid in elucidation functions. invaluable tools better functions pregnancy postnatal development.

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