Natural selection coupled with intragenic recombination shapes diversity patterns in the major histocompatibility complex class II genes of the giant panda.
作者： Yi-Yan Chen , Ying-Ying Zhang , He-Min Zhang , Yun-Fa Ge , Qiu-Hong Wan
DOI: 10.1002/JEZ.B.21327
关键词: Locus (genetics) 、 MHC Class II Gene 、 Genetic variation 、 Negative selection 、 Natural selection 、 Balancing selection 、 Major histocompatibility complex 、 Loss of heterozygosity 、 Genetics 、 Biology
摘要: Ample variations of the major histocompatibility complex (MHC) genes are essential for vertebrates to adapt various environmental conditions. In this study, we investigated genetic and evolutionary patterns seven functional MHC class II (one DRA, two DRB, DQA, DQB) giant panda. The results showed presence monomorphic loci (DRA DQB2) five polymorphic with different numbers alleles (seven at DRB1, six DRB3, DQA1, four DQA2, DQB1). balancing selection in panda was supported by following pieces evidence: (1) observed heterozygosity higher than expected. (2) Amino acid significantly antigen-binding sites (ABS) compared non-ABS sequences. (3) parameter ω (dN/dS) ABS (4) Approximately 95.45% positively selected codons (P>0.95) were located or adjacent an ABS. Furthermore, study that Qinling subspecies exhibited high values across each locus (all >1), supporting its extensive positive selection. Sichuan displayed small DRB1 (ω<0.72) DQA2 (ω<0.48), suggesting these underwent strong purifying Intragenic recombination detected DQB1. molecular diversity classic Aime-MHC implies had evolved relatively abundant adaptive immunity along history host–pathogen co-evolution. Collectively, findings indicate natural accompanied drives contrasting between studied J. Exp. Zool. (Mol. Dev. Evol.) 314B:208–223, 2010. © 2009 Wiley-Liss, Inc.
sci-hub.se 参考文章(56)
Francois Rousset, None, genepop'007: a complete re-implementation of the genepop software for Windows and Linux Molecular Ecology Resources. ,vol. 8, pp. 103- 106 ,(2008) , 10.1111/J.1471-8286.2007.01931.X
S. Paterson, K. Wilson, J. M. Pemberton, Major histocompatibility complex variation associated with juvenile survival and parasite resistance in a large unmanaged ungulate population (Ovis aries L.) Proceedings of the National Academy of Sciences of the United States of America. ,vol. 95, pp. 3714- 3719 ,(1998) , 10.1073/PNAS.95.7.3714
Austin L. Hughes, Masatoshi Nei, Pattern of nucleotide substitution at major histocompatibility complex class I loci reveals overdominant selection Nature. ,vol. 335, pp. 167- 170 ,(1988) , 10.1038/335167A0
Chang-Jun Zeng, Hui-Juan Pan, Shao-Bin Gong, Jian-Qiu Yu, Qiu-Hong Wan, Sheng-Guo Fang, Giant panda BAC library construction and assembly of a 650-kb contig spanning major histocompatibility complex class II region BMC Genomics. ,vol. 8, pp. 315- 315 ,(2007) , 10.1186/1471-2164-8-315
H Westerdahl, H Wittzell, T von Schantz, S Bensch, MHC class I typing in a songbird with numerous loci and high polymorphism using motif-specific PCR and DGGE Heredity. ,vol. 92, pp. 534- 542 ,(2004) , 10.1038/SJ.HDY.6800450
M Amills, O Ramírez, A Tomàs, G Obexer-Ruff, O Vidal, Positive selection on mammalian MHC-DQ genes revisited from a multispecies perspective. Genes and Immunity. ,vol. 9, pp. 651- 658 ,(2008) , 10.1038/GENE.2008.62
H Schaschl, P Wandeler, F Suchentrunk, G Obexer-Ruff, S J Goodman, Selection and recombination drive the evolution of MHC class II DRB diversity in ungulates Heredity. ,vol. 97, pp. 427- 437 ,(2006) , 10.1038/SJ.HDY.6800892
Philip W Hedrick, Rhonda N Lee, Karen M Parker, Major histocompatibility complex (MHC) variation in the endangered Mexican wolf and related canids Heredity. ,vol. 85, pp. 617- 624 ,(2000) , 10.1046/J.1365-2540.2000.00805.X
P. Hořín, J. Matiašovic, A second locus and new alleles in the major histocompatibility complex class II (ELA-DQB) region in the horse Animal Genetics. ,vol. 33, pp. 196- 200 ,(2002) , 10.1046/J.1365-2052.2002.00839.X
HilaryC. Miller, DavidM. Lambert, Gene duplication and gene conversion in class II MHC genes of New Zealand robins (Petroicidae) Immunogenetics. ,vol. 56, pp. 178- 191 ,(2004) , 10.1007/S00251-004-0666-1