IAA-overproducer mutants of Hebeloma cylindrosporum Romagnesi mycorrhizal with Pinus pinaster (Ait.) Sol. and P. sylvestris L. in hydroponic culture

作者: Jan-Erik Nylund , Håkan Wallander , Björn Sundberg , Gilles Gay

DOI: 10.1007/BF00206772

关键词: PisolithusMycorrhizaLaccariaShootMutantEctomycorrhizaPetri dishPinus pinasterBotanyBiology

摘要: Indole-3-acetic acid (IAA) is thought to play a role in the regulation of ectomycorrhiza development, and vigorous mycorrhiza formers such as Pisolithus Laccaria have previously been shown accumulate large amounts IAA culture medium vitro, particularly presence tryptophan. Recently, 5-fluoroindole-resistant IAA-overproducing mutant strains Hebeloma cylindrosporum Romagnesi developed described by Durand et al. (1992). We used some these corresponding wild-type mycobionts on seedlings Pinus pinaster (Ait.) Sol. P. sylvestris L. semi-hydroponic an attempt study effects independent species-specific differences. However, no significant differences between were found host growth rate, shoot carbohydrate concentration, root morphology, concentration or mycorrhizal biomass. Since previous work showed stimulation other mutants formation Petri dish test tube cultures, we assume that system prevents build up tryptophan fungal origin, which most likely precondition for enhanced production.

参考文章(14)
Andreas Rieger, Martin Guttenberger, Rüdiger Hampp, Soluble Carbohydrates in Mycorrhized and Non-Mycorrhized Fine Roots of Spruce Seedlings Zeitschrift für Naturforschung C. ,vol. 47, pp. 201- 204 ,(1992) , 10.1515/ZNC-1992-3-406
Jan-Erik Nylund, Håkan Wallander, 5 Ergosterol Analysis as a Means of Quantifying Mycorrhizal Biomass Methods in Microbiology. ,vol. 24, pp. 77- 88 ,(1992) , 10.1016/S0580-9517(08)70088-6
G. Gay, R. Rouillon, J. Bernillon, J. Favre-Bonvin, IAA biosynthesis by the ectomycorrhizal fungus Hebeloma hiemale as affected by different precursors Botany. ,vol. 67, pp. 2235- 2239 ,(1989) , 10.1139/B89-285
V Slankis, Soil Factors Influencing Formation of Mycorrhizae Annual Review of Phytopathology. ,vol. 12, pp. 437- 457 ,(1974) , 10.1146/ANNUREV.PY.12.090174.002253
Jan‐Erik Nylund, The regulation of mycorrhiza formation— carbohydrate and hormone theories reviewed Scandinavian Journal of Forest Research. ,vol. 3, pp. 465- 479 ,(1988) , 10.1080/02827588809382532
H. Wallander, J. -E. Nylund, B. Sundberg, Ectomycorrhiza and nitrogen effects on root IAA: results contrary to current theory Mycorrhiza. ,vol. 1, pp. 91- 92 ,(1992) , 10.1007/BF00206142