Sequence analysis of linked maize 22 kDa alpha-zein genes.

作者: Gary A. Thompson , David R. Siemieniak , Leang C. Sieu , Jerry L. Slightom , Brian A. Larkins

DOI: 10.1007/BF00020030

关键词: BiologySequence analysisGeneSequence alignmentGene duplicationNucleic acid sequenceRegulatory sequenceMolecular biologyGeneticsHomology (biology)Pseudogene

摘要: We have determined the nucleotide sequence of a 7343 bp zein genomic clone (gZ22.8H3) from maize inbred W64A. Computer-aided analysis DNA revealed two contiguous 22 kDa α-zein genes. The 5′ gene (gZ22.8) encodes complete polypeptide and contains putative regulatory sequences in both 3′ flanking regions that are typical In contrast, (ψgZ22.8) appears to be pseudogene, because it numerous insertions deletions would prevent translation mRNA. Alignment genes indicated they resulted 3.3 kb duplication event.

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