Drosophila BLM in double-strand break repair by synthesis-dependent strand annealing.
作者： Melissa D Adams , Mitch McVey , Jeff J Sekelsky
关键词: DNA synthesis 、 Helicase 、 Double Strand Break Repair 、 Molecular biology 、 DNA 、 DNA repair 、 Homologous recombination 、 Bloom syndrome 、 DNA damage 、 Biology
摘要: Bloom syndrome, characterized by a predisposition to cancer, is caused mutation of the RecQ DNA helicase gene BLM. The precise function BLM remains unclear. Previous research suggested that Drosophila functions in repair double-strand breaks. Most breaks flies are repaired homologous recombination through synthesis-dependent strand-annealing pathway. Here, we demonstrate mutants severely impaired their ability carry out synthesis during strand annealing. Consequently, completed error-prone pathways create large deletions. These results suggest model which maintains genomic stability promoting efficient and thereby prevents break less pathways.
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