The draft genome of the pest tephritid fruit fly Bactrocera tryoni: resources for the genomic analysis of hybridising species.

摘要: The tephritid fruit flies include a number of economically important pests horticulture, with large accumulated body research on their biology and control. Amongst the Tephritidae, genus Bactrocera, containing over 400 species, presents various species groups potential utility for genetic studies speciation, behaviour or pest In Australia, there exists triad closely-related, sympatric Bactrocera which do not mate in wild but which, despite distinct morphologies behaviours, can be force-mated laboratory to produce fertile hybrid offspring. To exploit opportunities offered by genomics, such as efficient identification loci central earliest stages investigators require genomic resources future investigations. We produced draft de novo genome assembly Australia’s major tryoni. male (650 -700 Mbp) includes approximately 150Mb interspersed repetitive DNA sequences 60Mb satellite DNA. Assessment using conserved core eukaryotic indicated 98% completeness. Over 16,000 MAKER-derived gene models showed degree overlap other Dipteran reference genomes. sequence ribosomal RNA transcribed unit was also determined. Unscaffolded assemblies B. neohumeralis jarvisi were then produced; comparison tryoni that are more closely related than any Drosophila pair. similarity genomes exploited identify 4924 potentially diagnostic indels between all occur non-coding regions. This first resembles dipteran terms size putative coding sequences. For three included this study, we have identified comprehensive set non-redundant sequences, including unit, quantified families. These will facilitate further investigations mechanisms responsible behavioural morphological differences these tephritids. shown how whole data used generate simple tests very closely-related where only one is scaffolded.