High density binding of proteins and peptides to poly(D,L-lactide) grafted with polyacrylic acid.

摘要: The use of graft polymers for the functionalisation biomaterial surfaces is already widespread. We investigated adsorptive and covalent binding a variety proteins peptides to poly(D,L-lactide) grafted with polyacrylic acid. Covalent attachment was achieved through coupling amino groups protein/peptide carboxyl polymer by using water-soluble carbodiimide N-hydroxysuccinimide. Binding densities were determined automated acid analysis after hydrolysis both adsorbed covalently bound proteins. Experiments in absence presence reagents allow discriminate between binding. Although adsorptivc quite substantial absolute terms, amount protein relatively low as compared total protein. Total 20-30 microg/cm2 can easily be achieved. Depending on concentration properties peptides, 5% 80% totally may physically adsorbed. Densities expressed molecules/10 nm2 vary from 0.5 molecule fibronectin 2,000 laminin-peptide molecules: their clearly correlate respective molecular masses. Obviously, are governed individual three-dimensional space requirements rather than density available groups. From number groups/10 (18,000-30,000 COOH/10 nm2) average length acrylic molecules estimated. Based assumption that about 10 copolymer chains accommodated nm2, chains, which corresponds thickness phase, estimated 0.5-1 microm. organisation within phase further experiments (BSA) peptide (Val-Lys) allowed react either singular, consecutive or simultaneous way. Together XPS IR-ATR surface characterisation picture arrangement immobilised emerges.