Individual and combined effects of the rolA, B, and C genes on anthraquinone production in Rubia cordifolia transformed calli.

摘要: It is known that the rolA, rolB, and rolC genes of Agrobacterium rhizogenes T-DNA affect processes plant development activate synthesis secondary metabolites in transformed cells. Although a synergistic activity rol on root formation well-documented, little about their individual combined action metabolism. In present investigation, we provide evidence indicating are capable increasing biosynthesis anthraquinones (AQs) calli Rubia cordifolia. The stimulatory effect was due to increased transcription key gene AQ biosynthesis, isochorismate synthase (ICS) gene. strongest AQ-stimulating shown for an R. cordifolia culture expressing rolB at high levels, where ensured 15-fold increase accumulation compared with control, non-transformed calli. A tyrosine phosphatase inhibitor abolished rolB-induced production, thus involvement (de)phosphorylation rolB-mediated stimulation. rolA- rolC-expressing cultures produced 2.8- 4.3-fold higher levels AQs, respectively, when control However, not because rolA apparently attenuated biosynthesis. Therefore, rol-gene-mediated signals promote those which seem have point divergence. Biotechnol. Bioeng. 2008;100: 118–125. © 2007 Wiley Periodicals, Inc.