Discovery of novel biopreservation agents with inhibitory effects on growth of food-borne pathogens and their application to seafood products

作者: Ouissal Bourouni Chahad , Monia El Bour , Pilar Calo-Mata , Abdellatif Boudabous , Jorge Barros-Velàzquez

DOI: 10.1016/J.RESMIC.2011.08.005

关键词: Vibrio anguillarumBiopreservationBiologyFood spoilageListeria monocytogenesAeromonas hydrophilaAeromonas salmonicidaBacteriocinMicrobiologyPathogenic bacteria

摘要: Selection of protective cultures is relevant in order to biopreserve and improve the functional safety food products, mainly through inhibition spoilage and/or pathogenic bacteria. Accordingly, present study investigated potential applications lactic acid bacteria (LAB) biopreservation fish shellfish products. For this purpose, a collection 84 LAB strains isolated from sea bass (Dicentrarchus labrax) bream (Sparus aurata) was identified characterized for their inhibitory activities against most seafood-spoilage potentially commercial The bioactive belonged genus Enterococcus exhibited Carnobacterium sp, Bacillus Listeria monocytogenes, Aeromonas salmonicida, hydrophila Vibrio anguillarum. Treatment cell-free extracts with proteases revealed proteinaceous nature inhibition. Interestingly, containing bacteriocins remained 100% active after treatment up 100 °C 30 min or 121 15 min. Molecular analysis led identification investigated, including enterocins A, B, L50 P. All these proteins demonstrated remarkable anti-Listeria activity were found be heat-resistant small class IIa bacteriocins. results presented work open way minimally-processed seafood

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