In silico annotation of unreviewed acetylcholinesterase (AChE) in some lepidopteran insect pest species reveals the causes of insecticide resistance.

摘要: Abstract Lepidoptera is the second most diverse insect order outnumbered only by Coeleptera. Acetylcholinesterase (AChE) major target site for insecticides. Extensive use of insecticides, to inhibit function this enzyme, have resulted in development insecticide resistance. Complete knowledge proteins very important know cause Computational annotation acetylcholinesterase can be helpful characterization protein. fourteen lepidopteran pest species was annotated using different bioinformatics tools. AChE all hydrophilic and thermostable. All showed lower values instability index except L. orbonalis, S. exigua T. absoluta. Highest percentage Arg, Asp, Asn, Gln Cys were recorded P. rapae. High might reason resistance Phylogenetic analysis revealed absoluta, orbonalis are closely related emerged from same primary branch. Three functional motifs predicted eleven while two found followed secretory pathway signal peptides. No peptides exigua, absoluta follow non pathway. Arginine methylation cysteine palmotylation Glycosylphosphatidylinositol (GPI) anchor nine species.