Survival of Mammalian Cells Exposed to Pure Hypothermia in Culture

摘要: Publisher Summary This chapter discusses hypothermia in terms its applicability to any stress and of specific features. The exposure a cell the assessment final survival involve several distinct phases. Survival studies using colony-formation assay as function time temperature hypothermic conditions have been done on mammalian cells. demonstrates that four different lines Arrhenius plot inactivation (killing) shows break at temperatures well above zero similar for leakage hemoglobin out human red Membrane-bound enzymes membrane transport systems cease membranes undergo liquid crystalline-to-gel lipid phase transition. Even though it is observed weak breaks plots fluidity isolated plasmalemma V79 cells critical 8 30°C, because amount perturber cholesterol plasmalemma, probably doesn't completely solidify physiological although viscosity changes with reach point where Na–K pump may ions some even if adequate ATP are available.