Preliminary evaluation of in vitro prescreen assays for developmental toxicants based on cultured murine preimplantation embryos and a cell line developed from a bovine preimplantation embryo.
作者： B.W. Kemppainen , P. Terse , O. Zurovac , D. Stringfellow
DOI: 10.1016/0887-2333(96)00004-5
关键词: Blastocyst 、 Inner cell mass 、 In vitro toxicology 、 Embryo 、 Cell culture 、 Developmental toxicity 、 Immunology 、 Biology 、 Viability assay 、 Andrology 、 Embryonic stem cell
摘要: Abstract The purpose of this research was to evaluate in vitro assays and compare their efficiency accurate prediction the potential chemicals cause abnormal embryonic/foetal development. In were based on cultured murine preimplantation embryos a continuous cell line derived from bovine embryo. Preimplantation collected superovulated mice for 72 hr presence 10-fold dilutions coded compounds. embryonic development considered normal if hatched zona pellucida (with normal-appearing inner mass trophoblast cells) attached culture plate at end period. cells seeded 96-well plates, 24 control media, exposed 10- twofold test compounds hr, finally stained counted. inhibitory concentration (IC 50 ) lethal (LC concentrations (m m that decreased or viability by 50%, calculated three model 10–12 (known developmental toxicants non-toxicants). concordance between vivo animal toxicity data 83 87%, respectively. These accuracies are similar those other available assays, assay has added advantage being simple perform economical (about US$100 per assay).
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