Development of a Biosensor-Based Rapid Urine Test for Detection of Urogenital Schistosomiasis.

摘要: Schistosomiasis affects up to 300 million people in regions of South America, Southeast Asia, the Middle East, Mediterranean Europe, and sub-Saharan Africa. Chronic consequences schistosomiasis include anemia, physical cognitive retardation children, organ failure. With predilection for genitourinary tract, Schistosoma haematobium increases risk bladder cancer HIV infection women. Microscopic identification enumeration parasite eggs urine (S. haematobium) or stool (Schistosoma mansoni japonicum) has remained standard diagnosis [1]. Limitations microscopy need skilled personnel field with equipment its time-consuming nature. Particularly infrastructure-limited regions, point-of-care (POC) molecular diagnostics hold potential transform management infectious diseases such as that carry significant long-term morbidity if left undiagnosed. POC could allow selective drug administration individuals confirmed rather than entire schools communities, an integrated diagnosis–single dose therapy approach reduce costs campaigns minimize treatment-associated adverse effects. Electrochemical biosensors are well suited because their high sensitivity, low cost, ease integration into devices, portability reader instrumentation [2]. We have developed a strategy rapid (one hour) bacterial urinary tract infections using electrochemical [3,4]. Urinary cells lysed directly applied array sensors functionalized oligonucleotide probes targeting 16S ribosomal RNA (rRNA) common uropathogens Formation sequence-specific hybridization complex between pathogen rRNA labeled capture detector probe pairs is detected by enzyme tag mediates amperometric signal output (Fig 1). Fig 1 Biosensor-based detection pathogen. In this work, we demonstrate use our biosensor-based platform S. urine. them established strategy. After determining efficient egg lysis strategy, demonstrated direct spiked human