Dihydroartemisinin enhances VEGFR1 expression through up-regulation of ETS-1 transcription factor.
作者： Na Niu , Changmei Yu , Liqun Li , Qiang Liu , Wenqian Zhang
DOI: 10.7150/JCA.25082
关键词: Angiogenesis 、 Downregulation and upregulation 、 Vascular endothelial growth factor 、 RNA interference 、 Gene knockdown 、 Cell biology 、 Oncogene 、 Transcription (biology) 、 Umbilical vein 、 Chemistry
摘要: Angiogenesis is required for tumor growth. Dihydroartemisinin (DHA), a the effective anti-malarial derivative of artemisinin, demonstrated potent anti-angiogenic activities that closely related to regulation vascular endothelial growth factor (VEGF) signaling cascade. VEGF receptor 1 (VEGFR1), in cells (ECs), coordinately regulate angiogenic activity triggered by ligand-receptor binding. Here we aimed explore effects DHA on VEGFR1 expression ECs. We found significantly increases human umbilical vein (HUVECs). In addition, upregulates level V-Ets Avian Erythroblastosis Virus E26 Oncogene Homolog (ETS-1), transcriptional which binds promoter. ChIP assay showed ETS-1 binding -52 ETS motif Knockdown RNA interference abolished DHA-induced increase expression. Taken together, elevates via up-regulation transcription HUVECs.
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