Polypeptide complementary to peptides or proteins having an amino acid sequence or nucleotide coding sequence at least partially known

摘要: A method for determining the amino acid sequence of a polypeptide complementary to at least portion an original peptide or protein. In one aspect involves: (a) first nucleotide nucleic coding biosynthesis protein; (b) ascertaining second which base-pairs with acid, and acids pairing in antiparallel directions; (c) by when read same reading frame as sequence. The whose is thus determined may be obtained diverse means such as, example, chemical synthesis, derivation from protein larger containing said sequence, or, DNA, inserting into plasmid form recombinant DNA vector transforming unicellular organism therewith produce transformant biosynthesizing polypeptide. ascertainment particular sequences circumvented, aspect, utilizing relationships having hydropathies substitutions generally dictated base-pairing complementarity.