Tissue-specific regulation of the alpha-myosin heavy chain gene promoter in transgenic mice.

摘要: The intergenic region between the mouse alpha-myosin heavy chain (MHC) and beta-MHC genes was analyzed in terms of its ability to drive gene expression transgenic mice. Earlier, we reported that entire sufficient direct bacterial chloramphenicol acetyl transferase reporter a tissue-specific developmental stage-specific manner. Additional lines have been generated which include two deletions. first deletion, alpha-3, lacks distal 2.5 kilobase pairs upstream region, is competent tissue- developmental-specific transgene. A larger only 138 base transcriptional start site remain, shows no acetyltransferase activity either muscle or non-muscle tissue. Tissue surveys transgene indicated low levels lung, analyses via polymerase reaction confirmed presence endogenous alpha-MHC transcripts this Subsequently, an gene-specific riboprobe used detect cognate lung sections by situ hybridization. data show that, are localized thick intimal wall veins venules.