Synchronous circadian voltage rhythms with asynchronous calcium rhythms in the suprachiasmatic nucleus.

摘要: The suprachiasmatic nucleus (SCN), the master circadian clock, contains a network composed of multiple types neurons which are thought to form hierarchical and multioscillator system. molecular clock machinery in SCN drives membrane excitability sends time cue signals various brain regions peripheral organs. However, how at what day these transmit output remain largely unknown. Here, we successfully visualized voltage rhythms optically for many days using genetically encoded sensor, ArcLightD. Unexpectedly, synchronized across entire cultured slices, whereas simultaneously recorded Ca2+ topologically specific dorsal ventral regions. We further found that temporal order two is cell-type specific: phase-lead AVP but nearly phase VIP neurons. confirmed firing also synchronous coupled with rhythms. These results indicate networks asynchronous produce coherent