Mutually protective actions of kainic acid epileptic Preconditioning and sublethal global ischemia on hippocampal neuronal death: Involvement of adenosine A1 receptors and KATP channels

摘要: Preconditioning with sublethal ischemia attenuates the detrimental effects of subsequent prolonged ischemic insults. This research elucidates potential in vivo cross-tolerance between different neuronal death-generating treatments such as kainate administration, which induces seizures and global ischemia. study also investigates a mild epileptic insult on death rat hippocampus after subsequent, lethal stress using kainic acid (KA) model epilepsy. Three preconditioning groups were follows: group 1 was injected 5 mg/kg KA before 6-minute ischemia; 2 received 3-minute 7.5 KA; 3 5-mg/kg dose 7.5-mg/kg injection. The interval days. Neuronal degeneration, revealed by silver impregnation method analysis cresyl violet staining, markedly reduced rats preconditioned or treatment. Labeling terminal deoxynucleotidyl transferase-mediated 2′-deoxyuridine 5′triphosphate-biotin nick-end labeling DNA laddering confirmed component fragmentation neurons its reduction all animals. current supports existence bidirectional excitotoxicity suggests involvement adenosine A1 receptors sulfonylurea- ATP-sensitive K+ channels this protective phenomenon.