Upregulation of renin-angiotensin system during differentiation of monocytes to macrophages.

摘要: Background We have demonstrated that accumulated macrophages in human coronary arteries strongly express angiotensin converting enzyme accordance with the development of atheromatous plaques. However, there are few reports on regulation renin-angiotensin system and monocytes as their source. Objective To examine whether is upregulated during differentiation to macrophages, it further regulated by II cytokines. Materials methods used a leukemia cell line, THP-1, for monocytes. Differentiated induced adding phorbol 12-myristate 13-acetate 24 h, were macrophages. Expression messenger RNA components was measured quantitative reverse-transcriptase polymerase chain reaction. Angiotensin activity subtype-specific angiotensin-binding sites cultured cells, production culture medium measured. Results Macrophages expressed all except chymase. Cellular increased 3.2- 4.5-fold differentiation, respectively. type 1 (AT1) 2 (AT2) receptors 6.2-and 6.4-fold sustained 7 days. Incubation h caused downregulation both AT1 AT2 receptor RNA, but expression levels still more than threefold higher compared The density binding 0.26 +/- 0.02 0.15 0.01 fmol/10(6) Conclusion markedly activated monocyte/macrophage may participate atherosclerosis.