Sulfhydryl-maleimide crosslinking for enhancing catalytic activity and duration of biocatalyst

摘要: Abstract Hydrophilic bridging of an enzyme using sulfhydryl conjugation to produce aggregate is used enhance the activity and operational lifetime a glucose oxidase (GOx)-based biocatalyst. 1,8-Bismaleimido-diethyleneglycol (BMPEG) as alternative conventional cross-linker glutaraldehyde (GA), which suffers from downsides in terms catalytic safety. To evaluate effect adopting BMPEG cross-linker, reduced graphene oxide (RGO) iron phthalocyanine (FePc) composite (RGO/FePc) support material was under layer preparation [RGO/FePc]/[GOx-BMPEG] electrode for hydrogen peroxide oxidation reaction. Its electrochemical behavior verified by comparison with electrodes prepared GOx alone ([RGO/FePc]/GOx) GOx-GA ([RGO/FePc]/[GOx-GA]). exhibited higher sensitivity (62.4 μW mM−1 cm−2) longer operating duration (94.5% 3h) than [RGO/FePc]/[GOx-GA] (52.3 μW mM−1 cm-2, 67.4% 3 h), even though amount on found be around half that physiological condition (pH 7.4, 36.5 °C). The excellent performance attributed hydrophilicity cross-links, mitigates hindrance mass transfer typically caused hydrophobic cross-linkers such GA, bonding thiol groups BMPEG, protects inactivation. Thus, GOx-BMPEG superior use implantable bioelectronics.