Direct Recovery of Herpes Simplex Virus (HSV)-Specific T Lymphocyte Clones from Recurrent Genital HSV-2 Lesions

摘要: T lymphocytes were recovered from recurrent vesicular and ulcerative herpes simplex virus type 2 (HSV-2) lesions 4 patients cloned without exogenous secondary antigenic stimulation. Resultant cultures screened for antigen-specific proliferative cytotoxic responses. Of the 39 HSV-specific clones recovered, all CD3-positive; 38 CD4-positive 1 was CD8-positive. cell directly lesions, 6%-10% had responses, in contrast to a peripheral blood precursor frequency of CD4 cells approximately 1/10(3) 1/10(4) as measured by limiting dilution assays. CD4+ studied restricted HLA-DR, -DP, -DQ detected both type-common type-specific recovered. localize human genital HSV-2 can be obtained early lesions. The ability isolate allows study specificities functional properties tissue-resident lymphocytes.