Requirement of endogenous interferon-gamma production for resolution of Listeria monocytogenes infection.

摘要: Abstract Peritoneal exudate cells and splenic of mice infected with Listeria monocytogenes show increased production interferon-gamma (IFN-gamma) after antigen or mitogen stimulation. When an IFN-gamma-specific enzyme-linked immunosorbent assay was used, first observed 2 days infection in peritoneal 4 to 6 cells. The IFN-gamma correlated the clearance from cavity spleen. Macrophages derived at these times were activated as evidenced by expression nonspecific tumoricidal activity against 111In-labeled P815 mastocytoma Injection neutralizing monoclonal anti-IFN-gamma into 1-day-infected completely inhibited generation macrophages. Normal hamster IgG had no effect. In vivo, antibody also abrogated bacteria spleen cavity. Six injection a sublethal dose Listeria, control treated normal devoid contained less than 10(3) colony-forming units. However, carried more 8 X 10(6) units either anatomical site day exhibited higher mortality rate. These results indicate that is required for vivo macrophages during infection.